11/1/2022 0 Comments 4peaks sequence alignment software![]() ![]() The templates should be placed in column order as listed If you are submitting 20 or more samples, the template must be provided in a standard.Two primers equals two sequencing reactions, etc. One template/primer set equals one sequencing reaction. Provide 10 ul of the template for each reaction desired.Samples and primer must be dissolved in distilled water.Fragment Analysis: You submit fluorescent-labeled PCR fragments for analysis.Requirements for High-Throughput sequencing service The Genomics Core adds the sequencing reagents, cycles the plate, cleans the excessĭye from the reactions and runs the plate on the sequencer. High-Throughput sequencing service: Your sample and primer are submittedĪs a mixture for each sequencing reaction in a 96-well sequencer compatible plate.Requirements for Electrophoresis only service The Genomics Core runs your reactions on the sequencer. Electrophoresis only service: Your laboratory runs the sequencing reactionsĪnd removes excess dye.Template and primer requirements for Reaction & Electrophoresis service 4PEAKS SEQUENCE ALIGNMENT SOFTWARE FULLThis full service option is suggested when you have less than 15 samples to sequence. Reactions of excess Big Dye Terminator dyes, and run the reactions on the sequencer. The Genomics Core will set up the sequencing reactions, clean the Reaction & Electrophoresis service: Template and primer are submitted.Failure of sequencing reactions performed outside of GCF will be assumed to be the responsibility of the performing lab except in the event of an equipment malfunction.įour options are available for submitting samples to the Genomics Core for sequencing:.Re-enter the sample information on this web site and provide the new plate number to To request a rerun, provided enough sample and primer remain, the customer must Changing the templateĪnd/or primer constitutes a new sample submission for which you will be charged. All reruns requested by the customer of the same template and primer will be chargedįor the first and the second run if the second run is unsuccessful.Equipment failure, GCF reagent failure, or operator error causing sequenceįailure - sequence will be rerun at no additional charge.Big Dye Terminator Dye Removal at an additional cost of $2 per sample.Print request at an additional cost of $1 per sample.Expedited service at a cost of $50 per hour for sequencing outside of standard work hours. ![]()
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